Protein Engineering
Author | : Uwe T Bornscheuer |
Publisher | : |
Total Pages | : |
Release | : 2018-08-13 |
ISBN-10 | : 1493984632 |
ISBN-13 | : 9781493984633 |
Rating | : 4/5 (32 Downloads) |
Book excerpt: This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.