Book excerpt: This book highlights the current limitations of biogas production and yield and new avenues to improving them. Biogas production and yield are among the most important renewable energy targets for our world. Pursuing an innovative and biotechnological approach, the book presents alternative sources for biogas production and explores a broad range of aspects, including: pre-treatment of substrates, accelerators (enzyme-mediated) and inhibitors involved in the process of obtaining biogas and its yield, design specifications for digesters/modified digesters, managing biogas plants, microbial risk and slurry management, energy balance and positive climatic impacts of the biogas production chain, and the impacts on Human, Animal and Environmental Health (“One Health” concept for the biogas chain).